Effects of system peaks in ion pair reversed phase liquid chromatography for noscapine and metabolites
Summary
Type: HPLC Application #: 101220
Detection: UV 210nm Conditions: precolumn eluent:
1mM decyl sulphate in 5% (v/v) acetonitrile in phosphate buffer pH 2,
analytical column eluent:
2.5mM N,N-dimethyl-N-octylamine in 20% (v/v) acetonitrile in phosphate buffer pH 2; Authors: Johansson M Source: J Chromatogr 452 (1988) 241 - 255
Details
Legend: Chromatogram obtained by injection of 500µl of phosphate buffer pH 2 containing
the analytes,
noscapine,
narcotiline;
amounts injected:
A. noscapine 31 and narcotiline 23 pmol,
B. noscapine